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Pathotyping Escherichia coli by using miniaturized DNA microarrays.


ABSTRACT: The detection of virulence determinants harbored by pathogenic Escherichia coli is important for establishing the pathotype responsible for infection. A sensitive and specific miniaturized virulence microarray containing 60 oligonucleotide probes was developed. It detected six E. coli pathotypes and will be suitable in the future for high-throughput use.

SUBMITTER: Anjum MF 

PROVIDER: S-EPMC2042074 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature

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Pathotyping Escherichia coli by using miniaturized DNA microarrays.

Anjum Muna F MF   Mafura Muriel M   Slickers Peter P   Ballmer Karin K   Kuhnert Peter P   Woodward Martin J MJ   Ehricht Ralf R  

Applied and environmental microbiology 20070713 17


The detection of virulence determinants harbored by pathogenic Escherichia coli is important for establishing the pathotype responsible for infection. A sensitive and specific miniaturized virulence microarray containing 60 oligonucleotide probes was developed. It detected six E. coli pathotypes and will be suitable in the future for high-throughput use. ...[more]

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