Extended-spectrum-beta-lactamase-producing Enterobacteriaceae strains in various types of private health care centers.
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ABSTRACT:
SUBMITTER: Arpin C
PROVIDER: S-EPMC2043178 | biostudies-literature | 2007 Sep
REPOSITORIES: biostudies-literature
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Extended-spectrum-beta-lactamase-producing Enterobacteriaceae strains in various types of private health care centers.
Arpin Corinne C Coulange Laure L Dubois Véronique V André Catherine C Fischer Isabelle I Fourmaux Sophie S Grobost Frédéric F Jullin Jacqueline J Dutilh Brigitte B Couture Jean-Francois JF Noury Patrick P Lagrange Isabelle I Ducastaing Aline A Doermann Henri-Pierre HP Quentin Claudine C
Antimicrobial agents and chemotherapy 20070625 9
During a 2004 survey, 49 extended-spectrum-beta-lactamase-producing enterobacteria were collected in 20 French private health care centers and one local hospital. They included 12 CTX-M-producing Escherichia coli strains (1.8% versus 0.3% in a 1999 survey). Most of them belonged to the same clone and contained a bla(CTX-M-15) gene on similar conjugative plasmids. ...[more]
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Project description:In 1999, 39 of 2,599 isolates of the family Enterobacteriaceae (1.5%) collected by eight private laboratories in the Aquitaine region in France produced an extended-spectrum beta-lactamase (ESBL). Among these were 19 Enterobacter aerogenes isolates; 8 Klebsiella pneumoniae isolates; 6 Escherichia coli isolates; 3 Proteus mirabilis isolates; and 1 isolate each of Serratia marcescens, Morganella morganii, and Providencia stuartii. ESBL producers were isolated from 38 patients, including 33 residents of 11 clinics or nursing homes and 5 ambulatory patients. Seven different ESBLs were characterized. These mainly consisted of TEM-24 (25 isolates) and TEM-21 (9 isolates), but TEM-15 (2 isolates) and TEM-3, TEM-19, SHV-4, and CTX-M-1 (1 isolate each) were also characterized. Seven strains showed the coexistence of different TEM- and/or SHV-encoding genes, including a new SHV-1 variant, SHV-44, defined by the substitution R205L previously reported for SHV-3 in association with S238G. The epidemiology of the ESBL producers was investigated by random amplification of polymorphic DNA, typing by enterobacterial repetitive intergenic consensus PCR, analysis of resistance cotransferred with the ESBL, and analysis of the restriction profiles of the ESBL-encoding plasmids. Of the TEM-24-expressing strains, 18 were E. aerogenes isolates, including 9 from the same clinic, that were representatives of the epidemic clone disseminating in France. Of the TEM-21-producing strains that belonged to different species of the family Enterobacteriaceae (E. coli, K. pneumoniae, and P. mirabilis), 8 were isolated in the same nursing home. Outbreaks due to strain and/or plasmid dissemination in these clinic and nursing home were demonstrated. The presence of ESBL producers in five ambulatory patients probably resulted from nosocomial acquisition. Our data highlight the serious need to monitor patients for ESBL-producing Enterobacteriaceae in general practice.
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Project description:Extended Spectrum Beta-Lactamase-producing Enterobacteriaceae Raw sequence reads
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