Stabilizing effect of Zn2+ in native bovine rhodopsin.
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ABSTRACT:
SUBMITTER: Park PS
PROVIDER: S-EPMC2043472 | biostudies-literature | 2007 Apr
REPOSITORIES: biostudies-literature
Publications
Stabilizing effect of Zn2+ in native bovine rhodopsin.
Park Paul S-H PS Sapra K Tanuj KT Koliński Michał M Filipek Sławomir S Palczewski Krzysztof K Muller Daniel J DJ
The Journal of biological chemistry 20070215 15
Single-molecule force spectroscopy (SMFS) is a powerful tool to dissect molecular interactions that govern the stability and function of proteins. We applied SMFS to understand the effect of Zn2+ on the molecular interactions underlying the structure of rhodopsin. Force-distance curves obtained from SMFS assays revealed the strength and location of molecular interactions that stabilize structural segments within this receptor. The inclusion of ZnCl2 in SMFS assay buffer increased the stability o ...[more]
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