Unknown

Dataset Information

0

MglA regulates Francisella tularensis subsp. novicida (Francisella novicida) response to starvation and oxidative stress.


ABSTRACT: MglA is a transcriptional regulator of genes that contribute to the virulence of Francisella tularensis, a highly infectious pathogen and the causative agent of tularemia. This study used a label-free shotgun proteomics method to determine the F. tularensis subsp. novicida (F. novicida) proteins that are regulated by MglA. The differences in relative protein amounts between wild-type F. novicida and the mglA mutant were derived directly from the average peptide precursor ion intensity values measured with the mass spectrometer by using a suite of mathematical algorithms. Among the proteins whose relative amounts changed in an F. novicida mglA mutant were homologs of oxidative and general stress response proteins. The F. novicida mglA mutant exhibited decreased survival during stationary-phase growth and increased susceptibility to killing by superoxide generated by the redox-cycling agent paraquat. The F. novicida mglA mutant also showed increased survival upon exposure to hydrogen peroxide, likely due to increased amounts of the catalase KatG. Our results suggested that MglA coordinates the stress response of F. tularensis and is likely essential for bacterial survival in harsh environments.

SUBMITTER: Guina T 

PROVIDER: S-EPMC2045168 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

MglA regulates Francisella tularensis subsp. novicida (Francisella novicida) response to starvation and oxidative stress.

Guina Tina T   Radulovic Dragan D   Bahrami Arya J AJ   Bolton Diana L DL   Rohmer Laurence L   Jones-Isaac Kendan A KA   Chen Jinzy J   Gallagher Larry A LA   Gallis Byron B   Ryu Soyoung S   Taylor Greg K GK   Brittnacher Mitchell J MJ   Manoil Colin C   Goodlett David R DR  

Journal of bacteriology 20070720 18


MglA is a transcriptional regulator of genes that contribute to the virulence of Francisella tularensis, a highly infectious pathogen and the causative agent of tularemia. This study used a label-free shotgun proteomics method to determine the F. tularensis subsp. novicida (F. novicida) proteins that are regulated by MglA. The differences in relative protein amounts between wild-type F. novicida and the mglA mutant were derived directly from the average peptide precursor ion intensity values mea  ...[more]

Similar Datasets

| S-EPMC4990168 | biostudies-literature
2008-12-31 | GSE11297 | GEO
| S-EPMC4069020 | biostudies-literature
| S-EPMC384726 | biostudies-literature
| PRJNA32143 | ENA
| PRJNA171329 | ENA
| PRJNA85571 | ENA
2022-05-31 | GSE174793 | GEO
| S-EPMC2569856 | biostudies-literature
| S-EPMC2805214 | biostudies-literature