Evaluation of pan-dermatophyte nested PCR in diagnosis of onychomycosis.
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ABSTRACT:
SUBMITTER: Garg J
PROVIDER: S-EPMC2045349 | biostudies-literature | 2007 Oct
REPOSITORIES: biostudies-literature
Publications
Evaluation of pan-dermatophyte nested PCR in diagnosis of onychomycosis.
Garg Jaya J Tilak Ragini R Singh Sanjay S Gulati Anil Kumar AK Garg Atul A Prakash Pradyot P Nath Gopal G
Journal of clinical microbiology 20070815 10
In this study, nested PCR using novel primers targeting the pan-dermatophyte-specific sequence of the chitin synthase 1 gene (CHS1) was compared with KOH microscopy, culture isolation, and single-round PCR for diagnosis of 152 patients with clinically suspected onychomycosis. Results indicate that nested PCR may be considered the gold standard for the diagnosis of cases of onychomycosis for which the etiological agents are dermatophytes. ...[more]
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