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Different activities of the largest subunit of replication protein A cooperate during SV40 DNA replication.

ABSTRACT: Replication protein A (RPA) is a stable heterotrimeric complex consisting of p70, p32 and p14 subunits. The protein plays a crucial role in SV40 minichromosome replication. Peptides of p70 representing interaction sites for the smaller two subunits, DNA as well as the viral initiator protein large T-antigen (Tag) and the cellular DNA polymerase alpha-primase (Pol) all interfered with the replication process indicating the importance of the different p70 activities in this process. Inhibition by the peptide disrupting protein-protein interactions was observed only during the pre-initiation stage prior to primer synthesis, suggesting the formation of a stable initiation complex between RPA, Tag and Pol at the primer end.

SUBMITTER: Taneja P

PROVIDER: S-EPMC2045582 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature

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Different activities of the largest subunit of replication protein A cooperate during SV40 DNA replication.

Taneja Poonam P Boche Irene I Hartmann Hella H Nasheuer Heinz-Peter HP Grosse Frank F Fanning Ellen E Weisshart Klaus K

FEBS letters 20070725 21

Replication protein A (RPA) is a stable heterotrimeric complex consisting of p70, p32 and p14 subunits. The protein plays a crucial role in SV40 minichromosome replication. Peptides of p70 representing interaction sites for the smaller two subunits, DNA as well as the viral initiator protein large T-antigen (Tag) and the cellular DNA polymerase alpha-primase (Pol) all interfered with the replication process indicating the importance of the different p70 activities in this process. Inhibition by ...[more]

PMID: 17673209

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