Protein-protein interactions among human lens acidic and basic beta-crystallins.
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ABSTRACT:
SUBMITTER: Liu BF
PROVIDER: S-EPMC2045703 | biostudies-literature | 2007 Aug
REPOSITORIES: biostudies-literature
Publications
Protein-protein interactions among human lens acidic and basic beta-crystallins.
Liu Bing-Fen BF Liang Jack J-N JJ
FEBS letters 20070723 21
Human lens beta-crystallin contains four acidic (betaA1-->betaA4) and three basic (betaB1-->betaB3) subunits. They oligomerize in the lens, but it is uncertain which subunits are involved in the oligomerization. We used a two-hybrid system to detect protein-protein interactions systematically. Proteins were also expressed for some physicochemical studies. The results indicate that all acidic-basic pairs (betaA-betaB) except betaA4-betaBs pairs show strong hetero-molecular interactions. For acidi ...[more]
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