Ontology highlight
ABSTRACT:
SUBMITTER: van Haastert PJ
PROVIDER: S-EPMC2064281 | biostudies-literature | 2007 Jun
REPOSITORIES: biostudies-literature
van Haastert Peter J M PJ Keizer-Gunnink Ineke I Kortholt Arjan A
The Journal of cell biology 20070529 5
Chemotaxis toward different cyclic adenosine monophosphate (cAMP) concentrations was tested in Dictyostelium discoideum cell lines with deletion of specific genes together with drugs to inhibit one or all combinations of the second-messenger systems PI3-kinase, phospholipase C (PLC), phospholipase A2 (PLA2), and cytosolic Ca(2+). The results show that inhibition of either PI3-kinase or PLA2 inhibits chemotaxis in shallow cAMP gradients, whereas both enzymes must be inhibited to prevent chemotaxi ...[more]