Unknown

Dataset Information

0

Impact of environmental and genetic factors on biofilm formation by the probiotic strain Lactobacillus rhamnosus GG.


ABSTRACT: Lactobacillus rhamnosus GG (ATCC 53103) is one of the clinically best-studied probiotic organisms. Moreover, L. rhamnosus GG displays very good in vitro adherence to epithelial cells and mucus. Here, we report that L. rhamnosus GG is able to form biofilms on abiotic surfaces, in contrast to other strains of the Lactobacillus casei group tested under the same conditions. Microtiter plate biofilm assays indicated that in vitro biofilm formation by L. rhamnosus GG is strongly modulated by culture medium factors and conditions related to the gastrointestinal environment, including low pH; high osmolarity; and the presence of bile, mucins, and nondigestible polysaccharides. Additionally, phenotypic analysis of mutants affected in exopolysaccharides (wzb), lipoteichoic acid (dltD), and central metabolism (luxS) showed their relative importance in biofilm formation by L. rhamnosus GG.

SUBMITTER: Lebeer S 

PROVIDER: S-EPMC2074970 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Impact of environmental and genetic factors on biofilm formation by the probiotic strain Lactobacillus rhamnosus GG.

Lebeer Sarah S   Verhoeven Tine L A TL   Perea Vélez Mónica M   Vanderleyden Jos J   De Keersmaecker Sigrid C J SC  

Applied and environmental microbiology 20070907 21


Lactobacillus rhamnosus GG (ATCC 53103) is one of the clinically best-studied probiotic organisms. Moreover, L. rhamnosus GG displays very good in vitro adherence to epithelial cells and mucus. Here, we report that L. rhamnosus GG is able to form biofilms on abiotic surfaces, in contrast to other strains of the Lactobacillus casei group tested under the same conditions. Microtiter plate biofilm assays indicated that in vitro biofilm formation by L. rhamnosus GG is strongly modulated by culture m  ...[more]

Similar Datasets

| S-EPMC1797292 | biostudies-literature
2011-01-01 | GSE22536 | GEO
2011-01-01 | E-GEOD-22536 | biostudies-arrayexpress
| S-EPMC1932685 | biostudies-literature
| S-EPMC4990349 | biostudies-literature
| S-EPMC91031 | biostudies-literature
| S-EPMC9090826 | biostudies-literature
| S-EPMC3033674 | biostudies-literature
| S-EPMC3272942 | biostudies-literature
| S-EPMC3623246 | biostudies-literature