Ontology highlight
ABSTRACT:
SUBMITTER: Withers ST
PROVIDER: S-EPMC2075014 | biostudies-literature | 2007 Oct
REPOSITORIES: biostudies-literature
Withers Sydnor T ST Gottlieb Shayin S SS Lieu Bonny B Newman Jack D JD Keasling Jay D JD
Applied and environmental microbiology 20070810 19
We have developed a novel method to clone terpene synthase genes. This method relies on the inherent toxicity of the prenyl diphosphate precursors to terpenes, which resulted in a reduced-growth phenotype. When these precursors were consumed by a terpene synthase, normal growth was restored. We have demonstrated that this method is capable of enriching a population of engineered Escherichia coli for those clones that express the sesquiterpene-producing amorphadiene synthase. In addition, we enri ...[more]