Ontology highlight
ABSTRACT:
SUBMITTER: Klyachko O
PROVIDER: S-EPMC2075054 | biostudies-literature | 2007 Oct
REPOSITORIES: biostudies-literature
Klyachko Olga O Stein Barry D BD Grindle Nathan N Clay Keith K Fuqua Clay C
Applied and environmental microbiology 20070824 20
A Coxiella-type microbe occurs at 100% frequency in all Amblyomma americanum ticks thus far tested. Using laboratory-reared ticks free of other microbes, we identified the Amblyomma-associated Coxiella microbe in several types of tissue and at various stages of the life cycle of A. americanum by 16S rRNA gene sequencing and diagnostic PCR. We visualized Amblyomma-associated Coxiella through the use of a diagnostic fluorescence in situ hybridization (FISH) assay supplemented with PCR-based detect ...[more]