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An Outbreak of Human Coronavirus OC43 Infection and Serological Cross-reactivity with SARS Coronavirus.


ABSTRACT: BACKGROUND:In summer 2003, a respiratory outbreak was investigated in British Columbia, during which nucleic acid tests and serology unexpectedly indicated reactivity for severe acute respiratory syndrome coronavirus (SARS-CoV). METHODS:Cases at a care facility were epidemiologically characterized and sequentially investigated for conventional agents of respiratory infection, SARS-CoV and other human CoVs. Serological cross-reactivity between SARS-CoV and human CoV-OC43 (HCoV-OC43) was investigated by peptide spot assay. RESULTS:Ninety-five of 142 residents (67%) and 53 of 160 staff members (33%) experienced symptoms of respiratory infection. Symptomatic residents experienced cough (66%), fever (21%) and pneumonia (12%). Eight residents died, six with pneumonia. No staff members developed pneumonia. Findings on reverse transcriptase-polymerase chain reaction assays for SARS-CoV at a national reference laboratory were suspected to represent false positives, but this was confounded by concurrent identification of antibody to N protein on serology. Subsequent testing by reverse transcriptase-polymerase chain reaction confirmed HCoV-OC43 infection. Convalescent serology ruled out SARS. Notably, sera demonstrated cross-reactivity against nucleocapsid peptide sequences common to HCoV-OC43 and SARS-CoV. CONCLUSIONS:These findings underscore the virulence of human CoV-OC43 in elderly populations and confirm that cross-reactivity to antibody against nucleocapsid proteins from these viruses must be considered when interpreting serological tests for SARS-CoV.

SUBMITTER: Patrick DM 

PROVIDER: S-EPMC2095096 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature

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An Outbreak of Human Coronavirus OC43 Infection and Serological Cross-reactivity with SARS Coronavirus.

Patrick David M DM   Petric Martin M   Skowronski Danuta M DM   Guasparini Roland R   Booth Timothy F TF   Krajden Mel M   McGeer Patrick P   Bastien Nathalie N   Gustafson Larry L   Dubord Janet J   Macdonald Diane D   David Samara T ST   Srour Leila F LF   Parker Robert R   Andonov Anton A   Isaac-Renton Judith J   Loewen Nadine N   McNabb Gail G   McNabb Alan A   Goh Swee-Han SH   Henwick Scott S   Astell Caroline C   Guo Jian Ping JP   Drebot Michael M   Tellier Raymond R   Plummer Francis F   Brunham Robert C RC  

The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale 20061101 6


<h4>Background</h4>In summer 2003, a respiratory outbreak was investigated in British Columbia, during which nucleic acid tests and serology unexpectedly indicated reactivity for severe acute respiratory syndrome coronavirus (SARS-CoV).<h4>Methods</h4>Cases at a care facility were epidemiologically characterized and sequentially investigated for conventional agents of respiratory infection, SARS-CoV and other human CoVs. Serological cross-reactivity between SARS-CoV and human CoV-OC43 (HCoV-OC43  ...[more]

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