Ontology highlight
ABSTRACT:
SUBMITTER: Liu X
PROVIDER: S-EPMC21098 | biostudies-literature | 1998 Jul
REPOSITORIES: biostudies-literature
Liu X X Li P P Widlak P P Zou H H Luo X X Garrard W T WT Wang X X
Proceedings of the National Academy of Sciences of the United States of America 19980701 15
We report here the reconstitution of a pathway that leads to the apoptotic changes in nuclei by using recombinant DNA fragmentation factor (DFF), a heterodimeric protein of 40 and 45 kDa. Coexpression of DFF40 and DFF45 is required to generate recombinant DFF, which becomes activated when DFF45 is cleaved by caspase-3. The cleaved fragments of DFF45 dissociate from the DFF40, the active component of DFF. Purified DFF40 exhibited an intrinsic DNase activity that was markedly stimulated by chromat ...[more]