Ontology highlight
ABSTRACT:
SUBMITTER: Garstka HL
PROVIDER: S-EPMC212813 | biostudies-literature | 2003 Sep
REPOSITORIES: biostudies-literature
Garstka Heike L HL Schmitt Wolfgang E WE Schultz Jeanette J Sogl Bettina B Silakowski Barbara B Pérez-Martos Acisclo A Montoya Julio J Wiesner Rudolf J RJ
Nucleic acids research 20030901 17
Mitochondrial transcription factor A (TFAM) has been shown to stimulate transcription from mitochondrial DNA promoters in vitro. In order to determine whether changes in TFAM levels also regulate RNA synthesis in situ, recombinant human precursor proteins were imported into the matrix of rat liver mitochondria. After uptake of wt-TFAM, incorporation of [alpha-32P]UTP into mitochondrial mRNAs as well as rRNAs was increased 2-fold (P < 0.05), whereas import of truncated TFAM lacking 25 amino acids ...[more]