Ontology highlight
ABSTRACT:
SUBMITTER: Bianco PR
PROVIDER: S-EPMC2128729 | biostudies-literature | 2007 Nov
REPOSITORIES: biostudies-literature
Bianco Piero R PR Bradfield Justin J JJ Castanza Lauren R LR Donnelly Andrea N AN
Journal of molecular biology 20070922 3
Rad54 is a key component of the eukaryotic recombination machinery. Its presence in DNA strand-exchange reactions in vitro results in a significant stimulation of the overall reaction rate. Using untagged Rad54, we show that this stimulation can be attributed to enhancement of the formation of a key reaction intermediate known as DNA networks. Using a novel, single DNA molecule, dual-optical tweezers approach we show how Rad54 stimulates DNA network formation. We discovered that Rad54 oligomers ...[more]