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Unusual proliferation arrest and transcriptional control properties of a newly discovered E2F family member, E2F-6.

ABSTRACT: E2F transcription factors play an important role in the regulation of cell cycle progression. We report here the cloning and characterization of an additional member of this family, E2F-6. E2F-6 lacks pocket protein binding and transactivation domains, and it is a potent transcriptional repressor that contains a modular repression domain at its carboxyl terminus. Overproduction of E2F-6 had no specific effect on cell cycle progression in asynchronously growing Saos2 and NIH 3T3 cells, but it inhibited entry into S phase of NIH 3T3 cells stimulated to exit G0. Taken together, these data suggest that E2F-6 can regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression.

SUBMITTER: Gaubatz S

PROVIDER: S-EPMC21314 | biostudies-literature | 1998 Aug

REPOSITORIES: biostudies-literature

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Unusual proliferation arrest and transcriptional control properties of a newly discovered E2F family member, E2F-6.

Gaubatz S S Wood J G JG Livingston D M DM

Proceedings of the National Academy of Sciences of the United States of America 19980801 16

E2F transcription factors play an important role in the regulation of cell cycle progression. We report here the cloning and characterization of an additional member of this family, E2F-6. E2F-6 lacks pocket protein binding and transactivation domains, and it is a potent transcriptional repressor that contains a modular repression domain at its carboxyl terminus. Overproduction of E2F-6 had no specific effect on cell cycle progression in asynchronously growing Saos2 and NIH 3T3 cells, but it inh ...[more]

PMID: 9689056

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