Ontology highlight
ABSTRACT:
SUBMITTER: Hua XH
PROVIDER: S-EPMC2132576 | biostudies-literature | 1998 Jan
REPOSITORIES: biostudies-literature
The Journal of cell biology 19980101 2
Before initiation of DNA replication, origin recognition complex (ORC) proteins, cdc6, and minichromosome maintenance (MCM) proteins bind to chromatin sequentially and form preinitiation complexes. Using Xenopus laevis egg extracts, we find that after the formation of these complexes and before initiation of DNA replication, cdc6 is rapidly removed from chromatin, possibly degraded by a cdk2-activated, ubiquitin-dependent proteolytic pathway. If this displacement is inhibited, DNA replication fa ...[more]