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Prediction of a common beta-propeller catalytic domain for fructosyltransferases of different origin and substrate specificity.

ABSTRACT: The three-dimensional (3D) structure of fructan biosynthetic enzymes is still unknown. Here, we have explored folding similarities between reported microbial and plant enzymes that catalyze transfructosylation reactions. A sequence-structure compatibility search using TOPITS, SDP, 3D-PSSM, and SAM-T98 programs identified a beta-propeller fold with scores above the confidence threshold that indicate a structurally conserved catalytic domain in fructosyltransferases (FTFs) of diverse origin and substrate specificity. The predicted fold appeared related to that of neuraminidase and sialidase, of glycoside hydrolase families 33 and 34, respectively. The most reliable structural model was obtained using the crystal structure of neuraminidase (Protein Data Bank file: 5nn9) as template, and it is consistent with the location of previously identified functional residues of bacterial levansucrases (Batista et al., 1999; Song & Jacques, 1999). The sequence-sequence analysis presented here reinforces the recent inclusion of fungal and plant FTFs into glycoside hydrolase family 32, and suggests a modified sequence pattern H-x (2)-[PTV]-x (4)-[LIVMA]-[NSCAYG]-[DE]-P-[NDSC][GA]3 for this family.

SUBMITTER: Pons T

PROVIDER: S-EPMC2144480 | biostudies-literature | 2000 Nov

REPOSITORIES: biostudies-literature

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Prediction of a common beta-propeller catalytic domain for fructosyltransferases of different origin and substrate specificity.

Pons T T Hernández L L Batista F R FR Chinea G G

Protein science : a publication of the Protein Society 20001101 11

The three-dimensional (3D) structure of fructan biosynthetic enzymes is still unknown. Here, we have explored folding similarities between reported microbial and plant enzymes that catalyze transfructosylation reactions. A sequence-structure compatibility search using TOPITS, SDP, 3D-PSSM, and SAM-T98 programs identified a beta-propeller fold with scores above the confidence threshold that indicate a structurally conserved catalytic domain in fructosyltransferases (FTFs) of diverse origin and su ...[more]

PMID: 11305239

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Dataset Information

Prediction of a common beta-propeller catalytic domain for fructosyltransferases of different origin and substrate specificity.

Publications

Prediction of a common beta-propeller catalytic domain for fructosyltransferases of different origin and substrate specificity.

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