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Mitotic centromere-associated kinesin is important for anaphase chromosome segregation.

ABSTRACT: Mitotic centromere-associated kinesin (MCAK) is recruited to the centromere at prophase and remains centromere associated until after telophase. MCAK is a homodimer that is encoded by a single gene and has no associated subunits. A motorless version of MCAK that binds centromeres but not microtubules disrupts chromosome segregation during anaphase. Antisense-induced depletion of MCAK results in the same defect. MCAK overexpression induces centromere-independent bundling and eventual loss of spindle microtubule polymer suggesting that centromere-associated bundling and/or depolymerization activity is required for anaphase. Live cell imaging indicates that MCAK may be required to coordinate the onset of sister centromere separation.

SUBMITTER: Maney T

PROVIDER: S-EPMC2148171 | biostudies-literature | 1998 Aug

REPOSITORIES: biostudies-literature

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Mitotic centromere-associated kinesin is important for anaphase chromosome segregation.

Maney T T Hunter A W AW Wagenbach M M Wordeman L L

The Journal of cell biology 19980801 3

Mitotic centromere-associated kinesin (MCAK) is recruited to the centromere at prophase and remains centromere associated until after telophase. MCAK is a homodimer that is encoded by a single gene and has no associated subunits. A motorless version of MCAK that binds centromeres but not microtubules disrupts chromosome segregation during anaphase. Antisense-induced depletion of MCAK results in the same defect. MCAK overexpression induces centromere-independent bundling and eventual loss of spin ...[more]

PMID: 9700166

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