Transposition of two amino acids changes a promiscuous RNA binding protein into a sequence-specific RNA binding protein.
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ABSTRACT:
SUBMITTER: Garrey SM
PROVIDER: S-EPMC2151040 | biostudies-literature | 2008 Jan
REPOSITORIES: biostudies-literature
Publications
Transposition of two amino acids changes a promiscuous RNA binding protein into a sequence-specific RNA binding protein.
Garrey Stephen M SM Cass Danielle M DM Wandler Anica M AM Scanlan Mary S MS Berglund J Andrew JA
RNA (New York, N.Y.) 20071113 1
In yeast (Saccharomyces cerevisiae), the branchpoint binding protein (BBP) recognizes the conserved yeast branchpoint sequence (UACUAAC) with a high level of specificity and affinity, while the human branchpoint binding protein (SF1) binds the less-conserved consensus branchpoint sequence (CURAY) in human introns with a lower level of specificity and affinity. To determine which amino acids in BBP provide the additional specificity and affinity absent in SF1, a panel of chimeric SF1 proteins was ...[more]
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