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Hypoxia regulates the expression of fatty acid-binding proteins in primary term human trophoblasts.
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ABSTRACT: Objective
Fatty acids (FAs) are essential for fetal development. Cellular FA uptake is modulated by fatty acid-binding proteins (FABPs). We hypothesized that hypoxia regulates the expression of FABPs in human trophoblasts.Study design
Primary term human trophoblasts were cultured for 72 hours in either standard (O2 = 20%) or hypoxic (O2 < 1%) conditions. FABP expression was interrogated using polymerase chain reaction and Western immunoblotting. Trophoblast lipid droplets were examined using dipyrromethene boron difluoride 493/503 staining.Results
We detected the expression of FABP1, -3, -4, -5, and pm but not FABP2 or FABP6-9 subtypes in trophoblasts. Exposure to hypoxia markedly increased lipid droplet accumulation in trophoblasts. Consistent with this observation, hypoxia enhanced the expression of FABP1, -3, and -4. Lastly, agonists of peroxisome proliferator-activated receptor-gamma enhanced the expression of FABP1 and -4 in trophoblasts.Conclusion
Hypoxia enhances the expression of FABP1, -3, and -4 in term human trophoblasts, suggesting that FABPs support fat accumulation in the hypoxic placenta.
SUBMITTER: Biron-Shental T
PROVIDER: S-EPMC2151846 | biostudies-literature | 2007 Nov
REPOSITORIES: biostudies-literature
Publications
Hypoxia regulates the expression of fatty acid-binding proteins in primary term human trophoblasts.
American journal of obstetrics and gynecology 20070910 5
<h4>Objective</h4>Fatty acids (FAs) are essential for fetal development. Cellular FA uptake is modulated by fatty acid-binding proteins (FABPs). We hypothesized that hypoxia regulates the expression of FABPs in human trophoblasts.<h4>Study design</h4>Primary term human trophoblasts were cultured for 72 hours in either standard (O2 = 20%) or hypoxic (O2 < 1%) conditions. FABP expression was interrogated using polymerase chain reaction and Western immunoblotting. Trophoblast lipid droplets were ex ...[more]