Molecular dissection of a bifidobacterial replicon.
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ABSTRACT:
SUBMITTER: Cronin M
PROVIDER: S-EPMC2168138 | biostudies-literature | 2007 Dec
REPOSITORIES: biostudies-literature
Publications
Molecular dissection of a bifidobacterial replicon.
Cronin Michelle M Knobel Moritz M O'Connell-Motherway Mary M Fitzgerald Gerald F GF van Sinderen Douwe D
Applied and environmental microbiology 20071026 24
The 2.1-kb cryptic plasmid pCIBAO89 from Bifidobacterium asteroides harbors a 1.4-kb segment which is sufficient for its autonomous replication. The segment is divided into two parts, the presumed replication origin, ori89, and the rep gene encoding the putative 41-kDa Rep89 replication initiation protein. This minimal replication region of pCIBAO89 was functionally dissected by transcriptional analyses as well as by DNA-binding studies, and the information obtained was exploited to create a num ...[more]
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