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Reconstitution of glyphosate resistance from a split 5-enolpyruvyl shikimate-3-phosphate synthase gene in Escherichia coli and transgenic tobacco.

ABSTRACT: A highly N-phosphonomethylglycine (glyphosate)-resistant Pseudomonas fluorescens G2 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) was mapped to identify potential split sites using a transposon-based linker-scanning procedure. Intein-mediated protein complementation was used to reconstitute glyphosate resistance from the genetically divided G2 EPSPS gene in Escherichia coli strain ER2799 and transgenic tobacco.

SUBMITTER: Dun BQ 

PROVIDER: S-EPMC2168149 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature

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Reconstitution of glyphosate resistance from a split 5-enolpyruvyl shikimate-3-phosphate synthase gene in Escherichia coli and transgenic tobacco.

Dun Bao-Qing BQ   Wang Xu-Jing XJ   Lu Wei W   Zhao Zhong-Lin ZL   Hou Song-Na SN   Zhang Bao-Ming BM   Li Gui-Ying GY   Evans Thomas C TC   Xu Ming-Qun MQ   Lin Min M  

Applied and environmental microbiology 20071019 24

A highly N-phosphonomethylglycine (glyphosate)-resistant Pseudomonas fluorescens G2 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) was mapped to identify potential split sites using a transposon-based linker-scanning procedure. Intein-mediated protein complementation was used to reconstitute glyphosate resistance from the genetically divided G2 EPSPS gene in Escherichia coli strain ER2799 and transgenic tobacco. ...[more]

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