SNP discovery via 454 transcriptome sequencing.
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ABSTRACT:
SUBMITTER: Barbazuk WB
PROVIDER: S-EPMC2169515 | biostudies-literature | 2007 Sep
REPOSITORIES: biostudies-literature
Publications
SNP discovery via 454 transcriptome sequencing.
Barbazuk W Brad WB Emrich Scott J SJ Chen Hsin D HD Li Li L Schnable Patrick S PS
The Plant journal : for cell and molecular biology 20070727 5
A massively parallel pyro-sequencing technology commercialized by 454 Life Sciences Corporation was used to sequence the transcriptomes of shoot apical meristems isolated from two inbred lines of maize using laser capture microdissection (LCM). A computational pipeline that uses the POLYBAYES polymorphism detection system was adapted for 454 ESTs and used to detect SNPs (single nucleotide polymorphisms) between the two inbred lines. Putative SNPs were computationally identified using 260,000 and ...[more]
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