Ontology highlight
ABSTRACT:
SUBMITTER: Giordano R
PROVIDER: S-EPMC2174319 | biostudies-literature | 2000 Mar
REPOSITORIES: biostudies-literature
Giordano R R Magnano A R AR Zaccagnini G G Pittoggi C C Moscufo N N Lorenzini R R Spadafora C C
The Journal of cell biology 20000301 6
We show here that a reverse transcriptase (RT) activity is present in murine epididymal spermatozoa. Sperm cells incubated with human poliovirus RNA can take up exogenous RNA molecules and internalize them in nuclei. Direct PCR amplification of DNA extracted from RNA-incubated spermatozoa indicate that poliovirus RNA is reverse-transcribed in cDNA fragments. PCR analysis of two-cell embryos shows that poliovirus RNA-challenged spermatozoa transfer retrotranscribed cDNA molecules into eggs during ...[more]