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A kinesin-related protein, KRP(180), positions prometaphase spindle poles during early sea urchin embryonic cell division.

ABSTRACT: We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.

SUBMITTER: Rogers GC

PROVIDER: S-EPMC2175202 | biostudies-literature | 2000 Aug

REPOSITORIES: biostudies-literature

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A kinesin-related protein, KRP(180), positions prometaphase spindle poles during early sea urchin embryonic cell division.

Rogers G C GC Chui K K KK Lee E W EW Wedaman K P KP Sharp D J DJ Holland G G Morris R L RL Scholey J M JM

The Journal of cell biology 20000801 3

We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead ...[more]

PMID: 10931863

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