Unknown

Dataset Information

0

Heat-resistant DNA tile arrays constructed by template-directed photoligation through 5-carboxyvinyl-2'-deoxyuridine.


ABSTRACT: Template-directed DNA photoligation has been applied to a method to construct heat-resistant two-dimensional (2D) DNA arrays that can work as scaffolds in bottom-up assembly of functional biomolecules and nano-electronic components. DNA double-crossover AB-staggered (DXAB) tiles were covalently connected by enzyme-free template-directed photoligation, which enables a specific ligation reaction in an extremely tight space and under buffer conditions where no enzymes work efficiently. DNA nanostructures created by self-assembly of the DXAB tiles before and after photoligation have been visualized by high-resolution, tapping mode atomic force microscopy in buffer. The improvement of the heat tolerance of 2D DNA arrays was confirmed by heating and visualizing the DNA nanostructures. The heat-resistant DNA arrays may expand the potential of DNA as functional materials in biotechnology and nanotechnology.

SUBMITTER: Tagawa M 

PROVIDER: S-EPMC2175349 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature

altmetric image

Publications

Heat-resistant DNA tile arrays constructed by template-directed photoligation through 5-carboxyvinyl-2'-deoxyuridine.

Tagawa Miho M   Shohda Koh-ichiroh K   Fujimoto Kenzo K   Sugawara Tadashi T   Suyama Akira A  

Nucleic acids research 20071103 21


Template-directed DNA photoligation has been applied to a method to construct heat-resistant two-dimensional (2D) DNA arrays that can work as scaffolds in bottom-up assembly of functional biomolecules and nano-electronic components. DNA double-crossover AB-staggered (DXAB) tiles were covalently connected by enzyme-free template-directed photoligation, which enables a specific ligation reaction in an extremely tight space and under buffer conditions where no enzymes work efficiently. DNA nanostru  ...[more]

Similar Datasets

| S-EPMC166189 | biostudies-literature
| S-EPMC3083862 | biostudies-literature
| S-EPMC3985955 | biostudies-literature
| S-EPMC6123678 | biostudies-literature
| S-EPMC10113367 | biostudies-literature
| S-EPMC4072410 | biostudies-literature
| S-EPMC2475615 | biostudies-literature
| S-EPMC9209446 | biostudies-literature
| S-EPMC5312849 | biostudies-literature
| S-EPMC3548433 | biostudies-literature