Equilibrium dynamics of spermine-induced plasmid DNA condensation revealed by fluorescence lifetime correlation spectroscopy.
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ABSTRACT:
SUBMITTER: Humpolickova J
PROVIDER: S-EPMC2186250 | biostudies-literature | 2008 Feb
REPOSITORIES: biostudies-literature
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Equilibrium dynamics of spermine-induced plasmid DNA condensation revealed by fluorescence lifetime correlation spectroscopy.
Humpolícková Jana J Benda Ales A Sýkora Jan J Machán Radek R Kral Teresa T Gasinska Barbara B Enderlein Joerg J Hof Martin M
Biophysical journal 20071026 3
The spermine-induced DNA condensation is a first-order phase transition. Here, we apply a novel technique fluorescence lifetime correlation spectroscopy to analyze this transition in a greater detail. We show that the method allows for the observation of the condensed and uncondensed molecules simultaneously based solely on different fluorescence lifetimes of the intercalating fluorophore PicoGreen in the folded und unfolded domains of DNA. The auto- and cross-correlation functions reveal that a ...[more]
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