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Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor.

ABSTRACT: The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Ralpha chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Ralpha(-/)- mice, but did activate cells from gammac(-/)- mice. Thus, the functional TSLPR requires the IL-7Ralpha chain, but not the gammac chain for signaling.

SUBMITTER: Park LS

PROVIDER: S-EPMC2193276 | biostudies-literature | 2000 Sep

REPOSITORIES: biostudies-literature

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Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor.

Park L S LS Martin U U Garka K K Gliniak B B Di Santo J P JP Muller W W Largaespada D A DA Copeland N G NG Jenkins N A NA Farr A G AG Ziegler S F SF Morrissey P J PJ Paxton R R Sims J E JE

The Journal of experimental medicine 20000901 5

The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Ralpha chain cDNA resulted in conversion to high affinity bi ...[more]

PMID: 10974032

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Predictors of interleukin-33 and thymic stromal lymphopoietin levels in cord blood.

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| S-EPMC4471622 | biostudies-literature

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