Ontology highlight
ABSTRACT:
SUBMITTER: Ladame S
PROVIDER: S-EPMC2196205 | biostudies-literature | 2006 Feb
REPOSITORIES: biostudies-literature
Ladame Sylvain S Schouten James A JA Roldan Jose J Redman James E JE Neidle Stephen S Balasubramanian Shankar S
Biochemistry 20060201 5
We have recently described an engineered zinc finger protein (Gq1) that binds with high specificity to the intramolecular G-quadruplex formed by the human telomeric sequence 5'-(GGTTAG)(5)-3', and that inhibits the activity of the enzyme telomerase in vitro. Here we report site-directed mutagenesis, biophysical, and molecular modeling studies that provide new insights into quadruplex recognition by the zinc finger scaffold. We show that any one finger of Gq1 can be replaced with the correspondin ...[more]