Unknown

Dataset Information

0

The S2 subsites of cathepsins K and L and their contribution to collagen degradation.


ABSTRACT: The exchange of residues 67 and 205 of the S2 pocket of human cysteine cathepsins K and L induces a permutation of their substrate specificity toward fluorogenic peptide substrates. While the cathepsin L-like cathepsin K (Tyr67Leu/Leu205Ala) mutant has a marked preference for Phe, the Leu67Tyr/Ala205Leu cathepsin L variant shows an effective cathepsin K-like preference for Leu and Pro. A similar turnaround of inhibition was observed by using specific inhibitors of cathepsin K [1-(N-Benzyloxycarbonyl-leucyl)-5-(N-Boc-phenylalanyl-leucyl)carbohydrazide] and cathepsin L [N-(4-biphenylacetyl)-S-methylcysteine-(D)-Arg-Phe-beta-phenethylamide]. Molecular modeling studies indicated that mutations alter the character of both S2 and S3 subsites, while docking calculations were consistent with kinetics data. The cathepsin K-like cathepsin L was unable to mimic the collagen-degrading activity of cathepsin K against collagens I and II, DQ-collagens I and IV, and elastin-Congo Red. In summary, double mutations of the S2 pocket of cathepsins K (Y67L/L205A) and L (L67Y/A205L) induce a switch of their enzymatic specificity toward small selective inhibitors and peptidyl substrates, confirming the key role of residues 67 and 205. However, mutations in the S2 subsite pocket of cathepsin L alone without engineering of binding sites to chondroitin sulfate are not sufficient to generate a cathepsin K-like collagenase, emphasizing the pivotal role of the complex formation between glycosaminoglycans and cathepsin K for its unique collagenolytic activity.

SUBMITTER: Lecaille F 

PROVIDER: S-EPMC2203344 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

The S2 subsites of cathepsins K and L and their contribution to collagen degradation.

Lecaille Fabien F   Chowdhury Shafinaz S   Purisima Enrico E   Brömme Dieter D   Lalmanach Gilles G  

Protein science : a publication of the Protein Society 20070401 4


The exchange of residues 67 and 205 of the S2 pocket of human cysteine cathepsins K and L induces a permutation of their substrate specificity toward fluorogenic peptide substrates. While the cathepsin L-like cathepsin K (Tyr67Leu/Leu205Ala) mutant has a marked preference for Phe, the Leu67Tyr/Ala205Leu cathepsin L variant shows an effective cathepsin K-like preference for Leu and Pro. A similar turnaround of inhibition was observed by using specific inhibitors of cathepsin K [1-(N-Benzyloxycarb  ...[more]

Similar Datasets

| S-EPMC6292680 | biostudies-literature
| S-EPMC3760505 | biostudies-literature
| S-EPMC3819629 | biostudies-literature
| S-EPMC8565456 | biostudies-literature
| S-EPMC4522768 | biostudies-literature
| S-EPMC5917446 | biostudies-literature
| S-EPMC1149733 | biostudies-other
| S-EPMC1158374 | biostudies-other
| S-EPMC1183729 | biostudies-other
| S-EPMC3307885 | biostudies-literature