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Multiplexed genotyping of ABC transporter polymorphisms with the Bioplex suspension array.


ABSTRACT: We have developed and validated a consolidated bead-based genotyping platform, the Bioplex suspension array for simultaneous detection of multiple single nucleotide polymorphisms (SNPs) of the ATP-binding cassette transporters. Genetic polymorphisms have been known to influence therapeutic response and risk of disease pathologies. Genetic screening for therapeutic and diagnostic applications thus holds great promise in clinical management. The allele-specific primer extension (ASPE) reaction was used to assay 22 multiplexed SNPs for eight subjects. Comparison of the microsphere-based ASPE assay results to sequencing results showed complete concordance in genotype assignments. The Bioplex suspension array thus proves to be a reliable, cost-effective and high-throughput technological platform for genotyping. It can be easily adapted to customized SNP panels for specific applications involving large-scale mutation screening of clinically relevant markers.

SUBMITTER: Koo SH 

PROVIDER: S-EPMC2211573 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature

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Multiplexed genotyping of ABC transporter polymorphisms with the Bioplex suspension array.

Koo Seok Hwee SH   Ong Tan Ching TC   Chong Kok Ting KT   Lee Caroline Guat Lay CG   Chew Fook Tim FT   Lee Edmund Jon Deoon EJ  

Biological procedures online 20071101


We have developed and validated a consolidated bead-based genotyping platform, the Bioplex suspension array for simultaneous detection of multiple single nucleotide polymorphisms (SNPs) of the ATP-binding cassette transporters. Genetic polymorphisms have been known to influence therapeutic response and risk of disease pathologies. Genetic screening for therapeutic and diagnostic applications thus holds great promise in clinical management. The allele-specific primer extension (ASPE) reaction was  ...[more]

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