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Single-step biocompatible coating for sulfhydryl coupling of receptors using 2-(pyridinyldithio)ethylcarbamoyl dextran.

ABSTRACT: 2-(Pyridinyldithio)ethylcarbamoyl dextran (PDEC dextran) is developed herein as a novel biosensor coating material aimed for direct and facile fabrication of a sulfhydryl-specific capture surface on gold; the site-directed immobilization of a single free cysteine residue presented protein (human serum albumin, HSA) on PDEC dextran-coated surfaces without prior activation and subsequent specific binding of mouse monoclonal anti-HSA antibody to the resultant surface were demonstrated using surface plasmon resonance (SPR) assays.

SUBMITTER: Li X

PROVIDER: S-EPMC2213716 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature

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Single-step biocompatible coating for sulfhydryl coupling of receptors using 2-(pyridinyldithio)ethylcarbamoyl dextran.

Li Xin X Abell Christopher C Cooper Matthew A MA

Colloids and surfaces. B, Biointerfaces 20070703 1

2-(Pyridinyldithio)ethylcarbamoyl dextran (PDEC dextran) is developed herein as a novel biosensor coating material aimed for direct and facile fabrication of a sulfhydryl-specific capture surface on gold; the site-directed immobilization of a single free cysteine residue presented protein (human serum albumin, HSA) on PDEC dextran-coated surfaces without prior activation and subsequent specific binding of mouse monoclonal anti-HSA antibody to the resultant surface were demonstrated using surface ...[more]

PMID: 17703925

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