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Sirt1 deficiency attenuates spermatogenesis and germ cell function.

ABSTRACT: In mammals, Sirt1, a member of the sirtuin family of proteins, functions as a nicotinamide adenine dinucleotide-dependent protein deactylase, and has important physiological roles, including the regulation of glucose metabolism, cell survival, and mitochondrial respiration. The initial investigations of Sirt1 deficient mice have revealed a phenotype that includes a reduced lifespan, small size, and an increased frequency of abnormal sperm. We have now performed a detailed analysis of the molecular and functional effects of Sirt1 deficiency in the germ line of Sirt1 knock-out (-/-) mice. We find that Sirt1 deficiency markedly attenuates spermatogenesis, but not oogenesis. Numbers of mature sperm and spermatogenic precursors, as early as d15.5 of development, are significantly reduced ( approximately 2-10-fold less; P

SUBMITTER: Coussens M

PROVIDER: S-EPMC2216432 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature

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Sirt1 deficiency attenuates spermatogenesis and germ cell function.

Coussens Matthew M Maresh John G JG Yanagimachi Ryuzo R Maeda Gregg G Allsopp Richard R

PloS one 20080213 2

In mammals, Sirt1, a member of the sirtuin family of proteins, functions as a nicotinamide adenine dinucleotide-dependent protein deactylase, and has important physiological roles, including the regulation of glucose metabolism, cell survival, and mitochondrial respiration. The initial investigations of Sirt1 deficient mice have revealed a phenotype that includes a reduced lifespan, small size, and an increased frequency of abnormal sperm. We have now performed a detailed analysis of the molecul ...[more]

PMID: 18270565

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