Unknown

Dataset Information

0

Parvin-beta inhibits breast cancer tumorigenicity and promotes CDK9-mediated peroxisome proliferator-activated receptor gamma 1 phosphorylation.


ABSTRACT: Parvin-beta is a focal adhesion protein downregulated in human breast cancer cells. Loss of Parvin-beta contributes to increased integrin-linked kinase activity, cell-matrix adhesion, and invasion through the extracellular matrix in vitro. The effect of ectopic Parvin-beta expression on the transcriptional profile of MDA-MB-231 breast cancer cells, which normally do not express Parvin-beta, was evaluated. Particular emphasis was placed upon propagating MDA-MB-231 breast cancer cells in three-dimensional culture matrices. Interestingly, Parvin-beta reexpression in MDA-MB-231 cells increased the mRNA expression, serine 82 phosphorylation (mediated by CDK9), and activity of the nuclear hormone receptor peroxisome proliferator-activated receptor gamma (PPARgamma), and there was a concomitant increase in lipogenic gene expression as a downstream effector of PPARgamma. Importantly, Parvin-beta suppressed breast cancer growth in vivo, with associated decreased proliferation. These data suggest that Parvin-beta might influence breast cancer progression.

SUBMITTER: Johnstone CN 

PROVIDER: S-EPMC2223422 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Parvin-beta inhibits breast cancer tumorigenicity and promotes CDK9-mediated peroxisome proliferator-activated receptor gamma 1 phosphorylation.

Johnstone Cameron N CN   Mongroo Perry S PS   Rich A Sophie AS   Schupp Michael M   Bowser Mark J MJ   Delemos Andrew S AS   Tobias John W JW   Liu Yingqiu Y   Hannigan Gregory E GE   Rustgi Anil K AK  

Molecular and cellular biology 20071112 2


Parvin-beta is a focal adhesion protein downregulated in human breast cancer cells. Loss of Parvin-beta contributes to increased integrin-linked kinase activity, cell-matrix adhesion, and invasion through the extracellular matrix in vitro. The effect of ectopic Parvin-beta expression on the transcriptional profile of MDA-MB-231 breast cancer cells, which normally do not express Parvin-beta, was evaluated. Particular emphasis was placed upon propagating MDA-MB-231 breast cancer cells in three-dim  ...[more]

Similar Datasets

| S-EPMC26842 | biostudies-literature
| S-EPMC1592783 | biostudies-literature
| S-EPMC3981956 | biostudies-literature
| S-EPMC2903911 | biostudies-literature
| S-EPMC2612500 | biostudies-other
| S-EPMC6494719 | biostudies-literature
| S-EPMC6437553 | biostudies-literature
| S-EPMC1223802 | biostudies-other
| S-EPMC6777908 | biostudies-literature
| S-EPMC3044476 | biostudies-literature