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Detection of human papillomavirus type 31-neutralizing antibodies from naturally infected patients by an assay based on intracellular assembly of luciferase-expressing pseudovirions.


ABSTRACT: The aim of this study was to develop a highly sensitive human papillomavirus type 31 (HPV31) neutralization assay based on the production of pseudovirions carrying luciferase. Neutralizing antibodies against HPV31 were investigated in a set of HPV31 monoclonal antibodies and in women with evidence of HPV31 infection. Neutralizing antibodies were detected in 78% of subjects with a positive enzyme-linked immunosorbent assay.

SUBMITTER: Fleury MJ 

PROVIDER: S-EPMC2223855 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature

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Detection of human papillomavirus type 31-neutralizing antibodies from naturally infected patients by an assay based on intracellular assembly of luciferase-expressing pseudovirions.

Fleury Maxime J J MJ   Touzé Antoine A   de Sanjosé Silvia S   Bosch F Xavier FX   Klaustermeiyer Joellen J   Coursaget Pierre P  

Clinical and vaccine immunology : CVI 20071107 1


The aim of this study was to develop a highly sensitive human papillomavirus type 31 (HPV31) neutralization assay based on the production of pseudovirions carrying luciferase. Neutralizing antibodies against HPV31 were investigated in a set of HPV31 monoclonal antibodies and in women with evidence of HPV31 infection. Neutralizing antibodies were detected in 78% of subjects with a positive enzyme-linked immunosorbent assay. ...[more]

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