Ontology highlight
ABSTRACT:
SUBMITTER: Touze T
PROVIDER: S-EPMC2229476 | biostudies-literature | 2008 Jan
REPOSITORIES: biostudies-literature
Touzé Thierry T Tran An X AX Hankins Jessica V JV Mengin-Lecreulx Dominique D Trent M Stephen MS
Molecular microbiology 20071127 2
One-third of the lipid A found in the Escherichia coli outer membrane contains an unsubstituted diphosphate unit at position 1 (lipid A 1-diphosphate). We now report an inner membrane enzyme, LpxT (YeiU), which specifically transfers a phosphate group to lipid A, forming the 1-diphosphate species. (32)P-labelled lipid A obtained from lpxT mutants do not produce lipid A 1-diphosphate. In vitro assays with Kdo(2)-[4'-(32)P]lipid A as the acceptor shows that LpxT uses undecaprenyl pyrophosphate as ...[more]