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Borrelia burgdorferi basic membrane proteins A and B participate in the genesis of Lyme arthritis.


ABSTRACT: Lyme arthritis results from colonization of joints by Borrelia burgdorferi and the ensuing host response. Using gene array-based differential analysis of B. burgdorferi gene expression and quantitative reverse trancription-polymerase chain reaction, we identified two paralogous spirochete genes, bmpA and bmpB, that are preferentially up-regulated in mouse joints compared with other organs. Transfer of affinity-purified antibodies against either BmpA or BmpB into B. burgdorferi-infected mice selectively reduced spirochete numbers and inflammation in the joints. B. burgdorferi lacking bmpA/B were therefore generated to further explore the role of these proteins in the pathogenesis of Lyme disease. B. burgdorferi lacking bmpA/B were infectious in mice, but unable to persist in the joints, and they failed to induce severe arthritis. Complementation of the mutant spirochetes with a wild-type copy of the bmpA and bmpB genes partially restored the original phenotype. These data delineate a role for differentially produced B. burgdorferi antigens in spirochete colonization of mouse joints, and suggest new strategies for the treatment of Lyme arthritis.

SUBMITTER: Pal U 

PROVIDER: S-EPMC2234379 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature

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Borrelia burgdorferi basic membrane proteins A and B participate in the genesis of Lyme arthritis.

Pal Utpal U   Wang Penghua P   Bao Fukai F   Yang Xiuli X   Samanta Swapna S   Schoen Robert R   Wormser Gary P GP   Schwartz Ira I   Fikrig Erol E  

The Journal of experimental medicine 20071231 1


Lyme arthritis results from colonization of joints by Borrelia burgdorferi and the ensuing host response. Using gene array-based differential analysis of B. burgdorferi gene expression and quantitative reverse trancription-polymerase chain reaction, we identified two paralogous spirochete genes, bmpA and bmpB, that are preferentially up-regulated in mouse joints compared with other organs. Transfer of affinity-purified antibodies against either BmpA or BmpB into B. burgdorferi-infected mice sele  ...[more]

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