Protein kinase A, TOR, and glucose transport control the response to nutrient repletion in Saccharomyces cerevisiae.

ABSTRACT: Nutrient repletion leads to substantial restructuring of the transcriptome in Saccharomyces cerevisiae. The expression levels of approximately one-third of all S. cerevisiae genes are altered at least twofold when a nutrient-depleted culture is transferred to fresh medium. Several nutrient-sensing pathways are known to play a role in this process, but the relative contribution that each pathway makes to the total response has not been determined. To better understand this, we used a chemical-genetic approach to block the protein kinase A (PKA), TOR (target of rapamycin), and glucose transport pathways, alone and in combination. Of the three pathways, we found that loss of PKA produced the largest effect on the transcriptional response; however, many genes required both PKA and TOR for prop

SUBMITTER: Slattery MG 

PROVIDER: S-EPMC2238170 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature