Unknown

Dataset Information

0

Structure-function analysis of the RNA helicase maleless.


ABSTRACT: Loss of function of the RNA helicase maleless (MLE) in Drosophila melanogaster leads to male-specific lethality due to a failure of X chromosome dosage compensation. MLE is presumably involved in incorporating the non-coding roX RNA into the dosage compensation complex (DCC), which is an essential but poorly understood requirement for faithful targeting of the complex to the X chromosome. Sequence comparison predicts several RNA-binding domains in MLE but their properties have not been experimentally verified. We evaluated the RNA-binding characteristics of these conserved motifs and their contributions to RNA-stimulated ATPase activity, to helicase activity, as well as to the targeting of MLE to the nucleus and to the X chromosome territory. We find that RB2 is the dominant, conditional RNA-binding module, which is indispensable for ATPase and helicase activity whereas the N-terminal RB1 motif does not bind RNA, but is involved in targeting MLE to the X chromosome. The C-terminal domain containing a glycine-rich heptad repeat adds potential dimerization and RNA-binding surfaces which are not required for helicase activity.

SUBMITTER: Izzo A 

PROVIDER: S-EPMC2241912 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC5870758 | biostudies-literature
| S-EPMC5397179 | biostudies-literature
| S-EPMC5228290 | biostudies-literature
| S-EPMC102575 | biostudies-literature
| S-EPMC4873980 | biostudies-literature
| S-EPMC230244 | biostudies-other
| S-EPMC4211656 | biostudies-literature
| S-EPMC4733421 | biostudies-literature
| S-EPMC229362 | biostudies-literature
| S-EPMC10329254 | biostudies-literature