Ontology highlight
ABSTRACT:
SUBMITTER: Clark LA
PROVIDER: S-EPMC2242497 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
Clark Louis A LA Boriack-Sjodin P Ann PA Eldredge John J Fitch Christopher C Friedman Bethany B Hanf Karl J M KJ Jarpe Matthew M Liparoto Stefano F SF Li You Y Lugovskoy Alexey A Miller Stephan S Rushe Mia M Sherman Woody W Simon Kenneth K Van Vlijmen Herman H
Protein science : a publication of the Protein Society 20060405 5
Improving the affinity of a high-affinity protein-protein interaction is a challenging problem that has practical applications in the development of therapeutic biomolecules. We used a combination of structure-based computational methods to optimize the binding affinity of an antibody fragment to the I-domain of the integrin VLA1. Despite the already high affinity of the antibody (Kd approximately 7 nM) and the moderate resolution (2.8 A) of the starting crystal structure, the affinity was incre ...[more]