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The purification, crystallization and preliminary diffraction of a glycerophosphodiesterase from Enterobacter aerogenes.

ABSTRACT: The metallo-glycerophosphodiesterase from Enterobacter aerogenes (GpdQ) has been cloned, expressed in Escherichia coli and purified. Initial screening of crystallization conditions for this enzyme resulted in the identification of needles from one condition in a sodium malonate grid screen. Removal of the metals from the enzyme and subsequent optimization of these conditions led to crystals that diffracted to 2.9 angstroms and belonged to space group P2(1)3, with unit-cell parameter a = 164.1 angstroms. Self-rotation function analysis and V(M) calculations indicated that the asymmetric unit contains two copies of the monomeric enzyme, corresponding to a solvent content of 79%. It is intended to determine the structure of this protein utilizing SAD phasing from transition metals or molecular replacement.

SUBMITTER: Jackson CJ

PROVIDER: S-EPMC2242963 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature

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The purification, crystallization and preliminary diffraction of a glycerophosphodiesterase from Enterobacter aerogenes.

Jackson Colin J CJ Carr Paul D PD Kim Hye Kyung HK Liu Jian Wei JW Ollis David L DL

Acta crystallographica. Section F, Structural biology and crystallization communications 20060610 Pt 7

The metallo-glycerophosphodiesterase from Enterobacter aerogenes (GpdQ) has been cloned, expressed in Escherichia coli and purified. Initial screening of crystallization conditions for this enzyme resulted in the identification of needles from one condition in a sodium malonate grid screen. Removal of the metals from the enzyme and subsequent optimization of these conditions led to crystals that diffracted to 2.9 angstroms and belonged to space group P2(1)3, with unit-cell parameter a = 164.1 an ...[more]

PMID: 16820687

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