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Crystallization and preliminary X-ray diffraction analysis of a tRNASer acceptor-stem microhelix.

ABSTRACT: In order to understand elongator tRNA(Ser) and suppressor tRNA(Sec) identity elements, the respective acceptor-stem helices have been synthesized and crystallized in order to analyse and compare their structures in detail at high resolution. The synthesis, crystallization and preliminary X-ray diffraction results for a seven-base-pair tRNA(Ser) acceptor-stem helix are presented here. Diffraction data were collected to 1.8 A, applying synchrotron radiation and cryogenic cooling. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 36.14, b = 38.96, c = 30.81 A, beta = 110.69 degrees .

SUBMITTER: Forster C

PROVIDER: S-EPMC2243086 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray diffraction analysis of a tRNASer acceptor-stem microhelix.

Förster Charlotte C Krauss Norbert N Brauer Arnd B E AB Szkaradkiewicz Karol K Brode Svenja S Hennig Klaus K Fürste Jens P JP Perbandt Markus M Betzel Christian C Erdmann Volker A VA

Acta crystallographica. Section F, Structural biology and crystallization communications 20060531 Pt 6

In order to understand elongator tRNA(Ser) and suppressor tRNA(Sec) identity elements, the respective acceptor-stem helices have been synthesized and crystallized in order to analyse and compare their structures in detail at high resolution. The synthesis, crystallization and preliminary X-ray diffraction results for a seven-base-pair tRNA(Ser) acceptor-stem helix are presented here. Diffraction data were collected to 1.8 A, applying synchrotron radiation and cryogenic cooling. The crystals belo ...[more]

PMID: 16754981

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