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ABSTRACT:
SUBMITTER: Watanabe R
PROVIDER: S-EPMC2246616 | biostudies-literature | 2008 Jan
REPOSITORIES: biostudies-literature
Watanabe Rikiya R Iino Ryota R Shimabukuro Katsuya K Yoshida Masasuke M Noji Hiroyuki H
EMBO reports 20071207 1
F(1)-ATPase is a rotary molecular motor that makes 120 degrees stepping rotations, with each step being driven by a single-ATP hydrolysis. In this study, a new reaction intermediate of F(1)-ATPase was discovered at a temperature below 4 degrees C, which makes a pause at the same angle in its rotation as when ATP binds. The rate constant of the intermediate reaction was strongly dependent on temperature with a Q(10) factor of 19, implying that the intermediate reaction accompanies a large conform ...[more]