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Induction of L1 and L2 beta-lactamases of Stenotrophomonas maltophilia.

ABSTRACT: Isogenic L1 and L2 gene knockout mutants of Stenotrophomonas maltophilia KJ (KJDeltaL1 and KJDeltaL2, respectively) were constructed by xylE gene replacement. Induction kinetics of the L1 and L2 genes were evaluated by testing catechol 2,3-dioxygenase activity in the mutants. The results suggested that the induction of the L1 and L2 genes was differentially regulated.

SUBMITTER: Hu RM

PROVIDER: S-EPMC2258547 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature

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Induction of L1 and L2 beta-lactamases of Stenotrophomonas maltophilia.

Hu Rouh-Mei RM Huang Kuang-Jay KJ Wu Lii-Tzu LT Hsiao Ying-Ju YJ Yang Tsuey-Ching TC

Antimicrobial agents and chemotherapy 20071217 3

Isogenic L1 and L2 gene knockout mutants of Stenotrophomonas maltophilia KJ (KJDeltaL1 and KJDeltaL2, respectively) were constructed by xylE gene replacement. Induction kinetics of the L1 and L2 genes were evaluated by testing catechol 2,3-dioxygenase activity in the mutants. The results suggested that the induction of the L1 and L2 genes was differentially regulated. ...[more]

PMID: 18086856

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AmpDI is involved in expression of the chromosomal L1 and L2 beta-lactamases of Stenotrophomonas maltophilia.

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Project description:We sought to determine how a cystic fibrosis isolate of Stenotrophomonas maltophilia responds to relevant pH gradients (pH 5, 7, and 9) by growing the bacterium in phosphate buffered media and conducting RNAseq experiments. Our data suggests acidic conditions are stressful for strain FLR19, as it responded by increasing expression of stress-response and antibiotic-resistance genes.

2018-10-17 | GSE121347 | GEO

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| S-EPMC3056134 | biostudies-other

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