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Rapid DNA library construction for functional genomic and metagenomic screening.

ABSTRACT: A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample.

SUBMITTER: Schmitz JE 

PROVIDER: S-EPMC2258639 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature

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Rapid DNA library construction for functional genomic and metagenomic screening.

Schmitz Jonathan E JE   Daniel Anu A   Collin Mattias M   Schuch Raymond R   Fischetti Vincent A VA  

Applied and environmental microbiology 20071214 5

A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample. ...[more]

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