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Genome-wide, high-resolution DNA methylation profiling using bisulfite-mediated cytosine conversion.

ABSTRACT: Methylation of cytosines ((m)C) is essential for epigenetic gene regulation in plants and mammals. Aberrant (m)C patterns are associated with heritable developmental abnormalities in plants and with cancer in mammals. We have developed a genome-wide DNA methylation profiling technology employing a novel amplification step for DNA subjected to bisulfite-mediated cytosine conversion. The methylation patterns detected are not only consistent with previous results obtained with (m)C immunoprecipitation (mCIP) techniques, but also demonstrated improved resolution and sensitivity. The technology, named BiMP (for Bisulfite Methylation Profiling), is more cost-effective than mCIP and requires as little as 100 ng of Arabidopsis DNA.

SUBMITTER: Reinders J

PROVIDER: S-EPMC2259111 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature

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Genome-wide, high-resolution DNA methylation profiling using bisulfite-mediated cytosine conversion.

Reinders Jon J Delucinge Vivier Céline C Theiler Grégory G Chollet Didier D Descombes Patrick P Paszkowski Jerzy J

Genome research 20080124 3

Methylation of cytosines ((m)C) is essential for epigenetic gene regulation in plants and mammals. Aberrant (m)C patterns are associated with heritable developmental abnormalities in plants and with cancer in mammals. We have developed a genome-wide DNA methylation profiling technology employing a novel amplification step for DNA subjected to bisulfite-mediated cytosine conversion. The methylation patterns detected are not only consistent with previous results obtained with (m)C immunoprecipitat ...[more]

PMID: 18218979

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