Unknown

Dataset Information

0

Direct, continuous monitoring of air pollution by transgenic sensor mice responsive to halogenated and polycyclic aromatic hydrocarbons.


ABSTRACT: BACKGROUND:The aryl hydrocarbon receptor (AhR, also called the dioxin receptor) plays crucial roles in toxicologic responses of animals to environmental pollutants, especially to halogenated and polycyclic aromatic hydrocarbons. To achieve direct, continuous risk assessment of air pollution using biological systems, we generated transgenic sensor mice that produce secreted alkaline phosphatase (SEAP) under the control of AhR. METHODS:To characterize responses of the mice to AhR agonists, sensor mice were orally administered 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3-methylcholanthrene (3MC), benzo[a]pyrene (B[a]P), or beta-naphthoflavone (BNF), and serum levels of SEAP were evaluated. To monitor air pollution caused by cigarette smoke, we placed the mice each day in an experimental smoking room, and evaluated activity of serum SEAP for up to 4 days. Activation of AhR in individual organs was also examined by reverse transcription-polymerase chain reaction (RT-PCR) analysis of SEAP. RESULTS:In response to oral exposure to TCDD, sensor mice exhibited dramatic and sustained activation of AhR. The mice also responded sensitively to 3MC, B[a]P, and BNF. Activation of AhR was dose dependent, and the liver was identified as the main responding organ. After exposure to the smoking environment, sensor mice consistently exhibited transient, reversible activation of AhR. RT-PCR analysis of SEAP revealed that activation of AhR occurred predominantly in the lung. CONCLUSION:We are the first laboratory to demonstrate successfully direct, comprehensive monitoring of air pollution using genetically engineered mammals. The established system would be useful for real risk assessment of halogenated and polycyclic aromatic hydrocarbons in the air, especially in smoking environments.

SUBMITTER: Kasai A 

PROVIDER: S-EPMC2265056 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Direct, continuous monitoring of air pollution by transgenic sensor mice responsive to halogenated and polycyclic aromatic hydrocarbons.

Kasai Ayumi A   Hiramatsu Nobuhiko N   Hayakawa Kunihiro K   Yao Jian J   Kitamura Masanori M  

Environmental health perspectives 20080301 3


<h4>Background</h4>The aryl hydrocarbon receptor (AhR, also called the dioxin receptor) plays crucial roles in toxicologic responses of animals to environmental pollutants, especially to halogenated and polycyclic aromatic hydrocarbons. To achieve direct, continuous risk assessment of air pollution using biological systems, we generated transgenic sensor mice that produce secreted alkaline phosphatase (SEAP) under the control of AhR.<h4>Methods</h4>To characterize responses of the mice to AhR ag  ...[more]

Similar Datasets

| S-EPMC7037384 | biostudies-literature
| PRJNA590550 | ENA
| S-EPMC2830011 | biostudies-literature
| S-EPMC5634701 | biostudies-literature
2012-04-18 | GSE37326 | GEO
| S-EPMC3807510 | biostudies-literature
| S-EPMC5134738 | biostudies-literature
| PRJNA656884 | ENA
2012-04-17 | E-GEOD-37326 | biostudies-arrayexpress
| S-EPMC4764582 | biostudies-literature