Ontology highlight
ABSTRACT:
SUBMITTER: Chabert M
PROVIDER: S-EPMC2265149 | biostudies-literature | 2008 Mar
REPOSITORIES: biostudies-literature
Chabert Max M Viovy Jean-Louis JL
Proceedings of the National Academy of Sciences of the United States of America 20080303 9
We present a purely hydrodynamic method for the high-throughput encapsulation of single cells into picoliter droplets, and spontaneous self-sorting of these droplets. Encapsulation uses a cell-triggered Rayleigh-Plateau instability in a flow-focusing geometry, and self-sorting puts to work two extra hydrodynamic mechanisms: lateral drift of deformable objects in a shear flow, and sterically driven dispersion in a compressional flow. Encapsulation and sorting are achieved on-flight in continuous ...[more]