Unknown

Dataset Information

0

Immune responses against severe acute respiratory syndrome coronavirus induced by virus-like particles in mice.

ABSTRACT: Virus-like particles (VLPs) represent a promising vaccine against severe acute respiratory syndrome coronavirus (SARS CoV). In this study, recombinant baculovirus vAcS and vAcME were constructed to express the S protein and the M and E proteins of SARS CoV, respectively. Electron microscope analysis demonstrated the formation of VLPs in vAcME and vAcS coinfected insect cells. Mice immunized four times with VLPs developed high antibody titres against SARS CoV. In addition, VLPs elicited cell-mediated immunity as demonstrated by enhanced interferon-gamma and interleukin-4 production. VLPs also conferred protective immunity against the infection of Spike protein pseudotyped murine leukaemia virus. Our findings demonstrate that SARS CoV VLPs are immunogenic and can elicit strong SARS CoV-specific humoral and cellular immune responses in mice. This is the first study describing the immunogenicity of SARS CoV VLPs, providing valuable data for developing a protective vaccine against SARS CoV infection.

SUBMITTER: Lu X 

PROVIDER: S-EPMC2266036 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Immune responses against severe acute respiratory syndrome coronavirus induced by virus-like particles in mice.

Lu Xinya X   Chen Yao Y   Bai Bingke B   Hu Hui H   Tao Ling L   Yang Jihong J   Chen Jianjun J   Chen Ze Z   Hu Zhihong Z   Wang Hanzhong H  

Immunology 20070803 4

Virus-like particles (VLPs) represent a promising vaccine against severe acute respiratory syndrome coronavirus (SARS CoV). In this study, recombinant baculovirus vAcS and vAcME were constructed to express the S protein and the M and E proteins of SARS CoV, respectively. Electron microscope analysis demonstrated the formation of VLPs in vAcME and vAcS coinfected insect cells. Mice immunized four times with VLPs developed high antibody titres against SARS CoV. In addition, VLPs elicited cell-medi  ...[more]

Similar Datasets

Unknown Chimeric coronavirus-like particles carrying severe acute respiratory syndrome coronavirus (SCoV) S protein protect mice against challenge with SCoV.
| S-EPMC2267761 | biostudies-literature
Unknown Assembly of human severe acute respiratory syndrome coronavirus-like particles.
| S-EPMC7111196 | biostudies-literature
Unknown Humoral and cellular immune responses induced by 3a DNA vaccines against severe acute respiratory syndrome (SARS) or SARS-like coronavirus in mice.
| S-EPMC2620671 | biostudies-literature
Transcriptomics Severe acute respiratory syndrome coronavirus envelope protein regulates cell stress responses and apoptosis
2012-06-20 | E-GEOD-30589 | biostudies-arrayexpress
Unknown Genomic analysis reveals age-dependent innate immune responses to severe acute respiratory syndrome coronavirus.
| S-EPMC2546950 | biostudies-literature
Unknown Immune responses with DNA vaccines encoded different gene fragments of severe acute respiratory syndrome coronavirus in BALB/c mice.
| S-EPMC7092945 | biostudies-literature
Transcriptomics Severe acute respiratory syndrome coronavirus envelope protein regulates cell stress responses and apoptosis
2012-06-21 | GSE30589 | GEO
Unknown Humoral and Cellular Immune Responses Against Severe Acute Respiratory Syndrome Coronavirus 2 Variants and Human Coronaviruses After Single BNT162b2 Vaccination.
| S-EPMC8384414 | biostudies-literature
Unknown Severe Acute Respiratory Syndrome Coronavirus 2-Specific Antibody Responses in Coronavirus Disease Patients.
| S-EPMC7323511 | biostudies-literature
Unknown Biomimetic Virus-Like Particles as Severe Acute Respiratory Syndrome Coronavirus 2 Diagnostic Tools.
| S-EPMC7724985 | biostudies-literature